更新時(shí)間:2016-06-27
人小細(xì)胞肺癌;NCI-H2227,開(kāi)始培養(yǎng)保持細(xì)胞濃度在2×10 exp5個(gè)細(xì)胞/ ml和1×10 exp5和1×10 exp6個(gè)細(xì)胞/ ml之間。只用于實(shí)驗(yàn)室研究。不能被用于在臨床檢測(cè)和有關(guān)于人身安全的實(shí)驗(yàn)。
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人小細(xì)胞肺癌;NCI-H2227
形態(tài)特性 上皮樣
生長(zhǎng)特性 該細(xì)胞既有懸浮生長(zhǎng),又有貼壁生長(zhǎng)
特征特性人小細(xì)胞肺癌;NCI-H2227該細(xì)胞是1989年8月從一名54歲白人男性小細(xì)胞肺癌皮膚轉(zhuǎn)移灶中分離得到的。
培養(yǎng)條件 DMEM/F12(1:1): Ham's F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 5%FBS;0.005mg/ml 胰島素+0.01mg/ml 轉(zhuǎn)鐵蛋白+10nM β-雌二醇+10nM HEPES+2nM L-谷氨酰胺
傳代方法 1:2~1:4傳代;每周換液2次。
傳代情況
凍存條件 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
公司細(xì)胞廣泛用于國(guó)內(nèi)院校的細(xì)胞生物學(xué)研究,他們的很多論文都或多或少使用我們公司的細(xì)胞,作為實(shí)驗(yàn)項(xiàng)目研究
楊梅素 Myricetin X27491 "≥98% "
洋薊素/1,3-二咖啡酰奎寧酸 Cynarin X27492 "≥98% "
"葉黃素 " Xanthophyll X27493 "≥90% "
乙酰蒲公英萜醇 Taraxeryl acetate X27494 ≥90%
"乙酰紫草素 " Acetylshikonin X27495 "≥98% "
異佛手柑內(nèi)酯 Isobergapten X27496 ≥98%
異甘草苷 Isoliquiritin X27497 ≥98%
異甘草素 Isoliquiritigenin X27498 ≥98%
異古倫賓 Isocolumbin X27499 ≥98%
異葒草素 Isoorientin X27500 ≥98%
異茴芹內(nèi)酯 Isopimpinellin X27501 ≥95%
異蓮心堿 Isoliensinine X27502 98%
異綠原酸A(3,5-二咖啡酰奎寧酸) Isochlorogenic acid A(3',5') X27503 ≥96%
異綠原酸B(3,4-二咖啡酰奎寧酸) Isochlorogenic acid B(3,4') X27504 ≥97%
異綠原酸C(4,5-二咖啡酰奎寧酸) Isochlorogenic acid C(4,5') X27505 ≥97%
異麥角甾苷 Isoacteoside X27506 ≥98%
"異木香內(nèi)脂 " isoalantolactone X27507 "≥98% "
"異嗪皮啶 " Isofraxidin X27508 "≥98% "
異鼠李素-3-O-新橙皮糖苷 Isorhamnetin-3-O-neohesperidoside X27509 ≥98%
異水飛薊賓 Isosilybin (A+B) mixture X27510 ≥98%
異香蘭素 Isovanillin X27511 ≥98%
"茵陳色原酮 " Capillarisin X27512 "≥98% "
茵芋苷 Skimmin X27513 "≥98% "
淫羊藿次苷Ⅱ Icarisid Ⅱ X27514 95%
銀鍛苷 Tiliroside X27515 97%
銀杏內(nèi)酯C Gingkolide C X27516 "≥99% "
銀杏內(nèi)酯J Ginkgolide J X27517 "≥99% "
銀杏酸(C13:0) Ginkgolic Acid (C13:0) X27518 "≥98% "
銀杏酸(C17:1) Ginkgolic Acid (C17:1) X27519 "≥99% "
吲哚醇 Indolol X27520 "≥98% "
"隱綠原酸/4-咖啡酰奎寧酸 " 4-Dicaffeoylquinic Acid X27521 ≥97%
柚皮甙二氫查爾酮 Naringin dihydrochalcone X27522 ≥98%
柚皮素 Naringenin X27523 "≥98% "
右旋比靈堿 d-Bicuculline X27524 "≥98% "
右旋四氫巴馬汀 d-Tetrahydropalmatine X27525 "≥98% "
羽扇豆醇 Lupeol X27526 "≥98% "
育亨賓 Yohimbine X27527 ≥97%
"鳶尾苷元磺酸鈉 " Tectorigenin X27528 "≥98% "
鳶尾黃素 Tectorigenin X27529 ≥97%
"原花青素 " Procyanidin X27530 "≥98% "
"原花青素B2 " Procyanidin B2 X27531 "≥98% "
"原花青素B3 " Procyanidin B3 X27532 "≥98% "
原人參二醇(PPD) Protopanaxadiol X27533 ≥95%
原人參三醇(PPT) Protopanaxatriol X27534 ≥98%
原薯蕷皂苷 Protodioscin X27535 ≥98%
遠(yuǎn)志酸 Polygalacic acid X27536 ≥97%
遠(yuǎn)志皂苷元 Senegenin X27537 ≥96%
蕓香柚皮苷 Narirutin X27538 ≥98%
"澤蘭黃酮 " Hispidulin X27539 "≥98% "
"澤瀉醇B-23-醋酸酯 " AlisolB-23-acetate X27540 ≥97%
獐芽菜苦苷 Swertiamarine X27541 ≥98%
芝麻酚 Sesamol X27542 99%
知母皂苷A-Ⅲ Timosaponin A-Ⅲ X27543 ≥98%
知母皂苷元 Sarsasapogenin X27544 ≥98%
"竹葉香附素A " Raddeanin(Anemodeanin) A X27545 "≥98% "
紫草素 Alkannin X27546 ≥97%
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