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人小細胞肺癌細胞;NCI-H446

更新時間:2016-06-21

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人小細胞肺癌細胞;NCI-H446,開始培養保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關于人身安全的實驗。

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人小細胞肺癌細胞;NCI-H446
形態特性 上皮樣
生長特性 貼壁/懸浮生長,混合 
特征特性人小細胞肺癌細胞;NCI-H446該細胞是1982年由Carney D和Gazdar AF等從一位小細胞肺癌患者的胸腔積液中建立的。細胞的原始形態并不具有小細胞肺癌特征。這個細胞株是小細胞肺癌的生化和形態學上的變種,表達神經元*的烯醇酶和腦型肌酸激酶同工酶;左旋多巴脫羧酶、蠶素、抗利尿激素、催產素或胃泌激素釋放肽未達到可檢測水平。與正常細胞相比,該細胞c-myc DNA序列擴增約20倍,RNA增加15倍。zui初傳代培養基用含有5%FBS的RPMI1640,另外添加0.005 mg/ml胰島素、0.01 mg/ml轉鐵
培養條件 RPMI 1640 (w/o Hepes) 10%FBS 
傳代方法 1:3~1:9傳代;每周換液2~3次。
傳代情況 C5
凍存條件 基礎培養基+5%DMSO+20%FBS 
公司細胞廣泛用于國內院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究
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干擾素γ(IFNγ)檢測試劑盒(化學發光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17644 96T Oryctolagus cuniculus (Rabbit)
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粒細胞集落刺激因子(GCSF)檢測試劑盒(化學發光免疫分析法) CLIA Kit for Colony Stimulating Factor 3, Granulocyte (GCSF) X17646 96T Homo sapiens (Human)
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膠質細胞系來源神經營養因子(GDNF)檢測試劑盒(化學發光免疫分析法) CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) X17649 96T Mus musculus (Mouse)
膠質細胞系來源神經營養因子(GDNF)檢測試劑盒(化學發光免疫分析法) CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) X17650 96T Rattus norvegicus (Rat)
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Rho鳥嘌呤核苷酸交換因子25(ARHGEF25)檢測試劑盒(化學發光免疫分析法) CLIA Kit for Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) X17663 96T Homo sapiens (Human)
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嘌呤能受體P2X7(P2RX7)檢測試劑盒(化學發光免疫分析法) CLIA Kit for Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) X17696 96T Homo sapiens (Human)
絲聚蛋白(FLG)檢測試劑盒(化學發光免疫分析法) CLIA Kit for Filaggrin (FLG) X17697 96T Homo sapiens (Human)
 

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