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人結直腸癌細胞;T84

更新時間:2016-06-15

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人結直腸癌細胞;T84,開始培養保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關于人身安全的實驗。

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人結直腸癌細胞;T84
形態特性 上皮樣;多角 
生長特性 貼壁生長 
特征特性 人結直腸癌細胞;T84T84細胞來源于一位72歲的男性結直腸癌患者的肺部轉移灶;皮下接種至BALB/c裸鼠并連續傳代23次后建立該細胞系。在裸鼠身上傳代的過程中,該腫瘤仍保持原始的結直腸癌的組織學特性。T84細胞系生長至匯合狀態時形成單層細胞,相鄰細胞間有緊密連接和細胞橋粒;該細胞有多種肽類激素和神經遞質的受體,維持電解質的定向運輸;角蛋白陽性。
培養條件 DMEM/F12(1:1): Ham's F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS 
傳代方法 1:2~1:4傳代;每周2次。
傳代情況 C5
凍存條件 基礎培養基+5%DMSO+20%FBS
公司細胞廣泛用于國內院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究
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