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人慢性髓系白血病細胞;K562

更新時間:2016-06-16

簡要描述:

人慢性髓系白血病細胞;K562,開始培養保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關于人身安全的實驗。

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人慢性髓系白血病細胞;K562
形態特性 淋巴母細胞樣;圓形 
生長特性 懸浮生長 
特征特性人慢性髓系白血病細胞;K562該細胞是由Lozzio從一名53歲的慢性髓細胞性白血病急變期的女性患者的胸水中分離建立的。該細胞曾被認為來源于粒系,處于高度未分化階段;Anderson等人作了細胞膜特性的研究后,認為該細胞是紅白血病細胞系。該細胞是對自然殺傷細胞高度敏感的體外靶標,故而被廣泛應用于這方面的研究。K562的原始細胞是一種具有多向分化潛能的造血系統的惡性腫瘤細胞,能自發分化為紅系、粒系和單核系的可辨識的祖細胞。該細胞表達CD7(25%)。
培養條件 RPMI 1640 (w/o Hepes) 10%FBS 
傳代方法 維持細胞濃度在1×105~1×106/ml,每2~3天換液1次。
傳代情況 C5
凍存條件 基礎培養基+5%DMSO+20%FBS 
公司細胞廣泛用于國內院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究
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